Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.Features of Spectra Multicolor Broad Range Pr. Original Bio-Rad Prestained Protein Standards for SDS-PAGE are available in broad, low, and high ranges. Precision Plus Protein All Blue Prestained Protein Standards 1610373 Bio-Rad. SuperSignal West Femto Maximum Sensitivity Substrate. ![]() ![]() For colorimetric or chemiluminescence blot detection, StrepTactin-HRP or -AP conjugate is also needed. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Protein standards for western blotting applications selection guide. SeeBlue Plus2 Pre-Stained Standard contains 10 proteins (4250 kDa): 8 blue-dyed and 2 with contrasting colors, for easy and quick evaluation of electrophoresis and western transfer efficiency. Recombinant Precision Plus Protein Standards. Prestained Natural SDS-PAGE Protein Standards The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Bio-Rad's protein standards for western blotting applications. Lot-specific MWs are included with each vial. Well Number (Vol) 15 (15 µl) Gel percentage. Mini-PROTEAN TGX Precast Protein Gels Quick Start Guide Mini-PROTEAN Precast Protein Gels Instruction Manual and Application Guide. * MWs are of representative lots actual weights may vary. Protein Ladders and Standards (Markers) including unstained, prestained and western Supporting Documents. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on. Kaleidoscope Prestained Natural Standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots.Ĭalibrated Molecular Weights* of Kaleidoscope Protein Standards Protein Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. To optimize sample loading, see Determining the Appropriate Sample Load for Western Blots Protocol. Possible causes: Solutions: Sample overloading. Each lot of Kaleidoscope and SDS-PAGE prestained natural protein standards is individually calibrated for estimating the MW of sample proteins. If using a digital imager, you see regions highlighted as saturated. In addition to detection of specific proteins, the total protein in a sample can. A good, clean western relies on the specificity and sensitivity of your antibodies. Efficient protein transfer is required for maximum western blot sensitivity. Request sample Interactive selection tool. scn file for the actual Western Blot signal. These standards can be detected by fluorescence when excited at appropriate wavelengths.Prestained Nautral Standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. Separated proteins are transferred from the gel to a membrane where they are immobilized. I am recording my Western Blots on a Biorad Chemidoc System which results in one. ![]() Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10–250 kD). Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.
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